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Continuous biohydrogen production from diluted molasses in an anaerobic contact reactor

Sheng CHANG, Jianzheng LI, Feng LIU

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 140-148 doi: 10.1007/s11783-010-0258-2

摘要： An anaerobic contact reactor (ACR) system comprising a continuous flow stirred tank reactor (CSTR) with settler to decouple the hydraulic retention time (HRT) from solids retention time (SRT) was developed for fermentative hydrogen production from diluted molasses by mixed microbial cultures. The ACR was operated at various volumetric loading rates (VLRs) of 20–44 kgCOD·m ·d with constant HRT of 6 h under mesophilic conditions of 35°C. The SRT was maintained at about 46–50 h in the system. At the initial VLR of 20 kgCOD·m ·d , the hydrogen production rate dropped from 22.6 to 1.58 L·d as the hydrogen was consumed by the hydrogentrophic methanogen. After increasing the VLR to 28 kgCOD·m ·d and discharging the sludge for 6 consecutive times, the hydrogentrophic methanogens were eliminated, and the hydrogen content reached 36.4%. As the VLR was increased to 44 kgCOD·m ·d , the hydrogen production rate and hydrogen yield increased to 42.1 L·d and 1.40 mol H ·molglucose-consumed , respectively. The results showed that a stable ethanol-type fermentation that favored hydrogen production in the reactor was thus established with the sludge loading rate (SLR) of 2.0–2.5 kgCOD·kgMLVSS ·d . It was found that the ethanol increased more than other liquid fermentation products, and the ethanol/acetic acid (mol/mol) ratio increased from 1.27 to 2.45 when the VLR increased from 28 to 44 kgCOD·m ·d , whereas the hydrogen composition decreased from 40.4% to 36.4%. The results suggested that the anaerobic contact reactor was a promising bioprocess for fermentative hydrogen production.

关键词： fermentative hydrogen production     anaerobic contact reactor (ACR)     sludge loading rate (SLR)     butyric acid-type fermentation     ethanol-type fermentation    

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Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch

Shaojie Wang,Zhihong Ma,Ting Zhang,Meidan Bao,Haijia Su

《化学科学与工程前沿（英文）》 2017年 第11卷 第1期   页码 100-106 doi: 10.1007/s11705-017-1617-3

摘要： The production of bio-hydrogen from raw cassava starch via a mixed-culture dark fermentation process was investigated. The production yield of H was optimized by adjusting the substrate concentration and the microorganism mixture ratio. A maximum H yield of 1.72 mol H /mol glucose was obtained with a cassava starch concentration of 10 g/L to give a 90% utilization rate. The kinetics of the substrate utilization and of the generation of both hydrogen and volatile fatty acids were also investigated. The substrate utilization follows pseudo first order reaction kinetics, whereas the production of both H and the VFAs correlate with the Gompertz equation. These results show that cassava is a good candidate for the production of biohydrogen.

关键词： cassava     biohydrogen     mixed cultures     kinetics    

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Effects of nitrate concentration on biological hydrogen production by mixed cultures

Bo WANG, Wei WAN, Jianlong WANG,

《环境科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 380-386 doi: 10.1007/s11783-009-0142-0

摘要： The effects of nitrate on fermentative hydrogen production and soluble metabolites from mixed cultures were investigated by varying nitrate concentrations from 0 to 10g N/L at 35°C with an initial pH of 7.0. The results showed that the substrate degradation rate, hydrogen production potential, hydrogen yield, and average hydrogen production rate initially increased with increasing nitrate concentrations from 0 to 0.1g N/L, while they decreased with increasing nitrate concentrations from 0.1 to 10g N/L. The maximum hydrogen production potential of 305.0mL, maximum hydrogen yield of 313.1mL/g glucose, and maximum average hydrogen production rate of 13.3mL/h were obtained at a nitrate concentration of 0.1g N/L. The soluble metabolites produced by the mixed cultures contained only ethanol and acetic acid (HAc) without propionic acid (HPr) and butyric acid (HBu). This study used the Modified Logistic model to describe the progress of cumulative hydrogen production in batch tests. A concise model was proposed to describe the effects of nitrate concentration on average hydrogen production rate.

关键词： nitrogen source     biohydrogen     fermentative hydrogen production     quantitative kinetic model    

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Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus

LI Yongfeng, REN Nanqi, LI Jianzheng, LI Peng, YANG Chuanping

《能源前沿（英文）》 2007年 第1卷 第4期   页码 403-407 doi: 10.1007/s11708-007-0058-2

摘要： Anaerobic process of biohydrogen production is developed in this paper. The isolation and identification of high efficient biohydrogen production anaerobic bacteria are the important foundations for the fermented biohydrogen produc

关键词： fermented biohydrogen     high     important     identification     biohydrogen    

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Effect of illumination on the hydrogen-production capability of anaerobic activated sludge

Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO

《环境科学与工程前沿（英文）》 2012年 第6卷 第1期   页码 125-130 doi: 10.1007/s11783-011-0384-5

摘要： To investigate the influence of illumination on the fermentative hydrogen production system, the hydrogen production efficiencies of two kinds of anaerobic activated sludge (floc and granule) from an anaerobic baffled reactor were detected under visible light, dark and light-dark, respectively. The 10 mL floc sludge or granular sludge was respectively inoculated to 100 mL diluted molasses (chemical oxygen demand of 8000 mg·L ) in a 250 mL serum bottle, and cultured for 24 h at 37°C under different illumination conditions. The results showed that the floc was more sensitive to illumination than the granule. A hydrogen yield of 19.8 mL was obtained in the dark with a specific hydrogen production rate of 3.52 mol·kg MLVSS·d (floc), which was the highest among the three illumination conditions. Under dark condition, the hydrogen yield of floc sludge reached the highest with the specific hydrogen production rate of 3.52 mol·kg MLVSS·d , and under light-dark, light, the specific hydrogen production rate was 3.11 and 2.21 mol·kg MLVSS·d , respectively. The results demonstrated that the illumination may affect the dehydrogenase activity of sludge as well as the activity of hydrogen-producing acetogens and then impact hydrogen production capacity.

关键词： biohydrogen production     dark fermentation     anaerobic activated sludge     light     dehydrogenase    

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Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 513-521 doi: 10.1007/s11783-015-0777-y

摘要： The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production using anaerobic granular sludge as inoculum and, secondly, to study the metabolic shifts of an anaerobic community subjected to low partial gas pressures. With this goal in mind, seven different wastewater (four synthetic media, two industrial wastewater, and one domestic effluent) and the effect of applying vacuum on the systems were analyzed. The application of vacuum promoted an increase in the diversity of hydrogen-producing bacteria, such as , and promoted the dominance of acetoclastic- over hydrogenotrophic methanogens. The application of different media promoted a wide variety of metabolic pathways. Nevertheless, reduction of the hydrogen partial pressure by application of vacuum lead to further oxidation of reaction intermediates irrespective of the medium used, which resulted in higher hydrogen and methane production, and improved the COD removal. Interestingly, vacuum greatly promoted biogenic hydrogen production from a real wastewater, which opens possibilities for future application of dark fermentation systems to enhance biohydrogen yields.

关键词： dark fermentation     biohydrogen     wastewaters     vacuum    

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Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter

Guanghong ZHENG, Zhuhui KANG, Yifan QIAN, Lei WANG,

《环境科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 387-392 doi: 10.1007/s11783-009-0154-9

摘要： Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ. [J]. Front. Environ. Sci. Eng., 2016, 10(3): 513-521.Yongtao LV,Xuan CHEN,Lei WANG,Kai JU,Xiaoqiang CHEN,Rui MIAO,Xudong WANG. [J]. Front. Environ. Sci. Eng., 2016, 10(2): 390-398.Zulkifly JEMAAT,Josep Anton TORA,Albert BARTROLI,Julián CARRERA,Julio PEREZ. [J]. Front. Environ. Sci. Eng., 2015, 9(3): 528-533.Wei LI, Xiaowen DING, Min LIU, Yuewen GUO, Lei LIU. [J]. Front Envir Sci Eng, 2012, 6(6): 892-900.Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO. [J]. Front Envir Sci Eng, 2012, 6(1): 125-130.Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN. [J]. Front Envir Sci Eng Chin, 2011, 5(4): 519-525.Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG. [J]. Front Envir Sci Eng Chin, 2011, 5(2): 291-297.Yanhui ZHAN, Jianwei LIN, Yanling QIU, Naiyun GAO, Zhiliang ZHU. [J]. Front Envir Sci Eng Chin, 2011, 5(1): 65-75.Rongchang WANG, Xinmin ZHAN, Yalei ZHANG, Jianfu ZHAO. [J]. Front Envir Sci Eng Chin, 2011, 5(1): 48-56.Bo WANG, Wei WAN, Jianlong WANG, . [J]. Front.Environ.Sci.Eng., 2009, 3(4): 380-386.GUO Jinsong, YANG Guohong, FANG Fang, QIN Yu. [J]. Front.Environ.Sci.Eng., 2008, 2(4): 439-445.GENG Bing, ZHU Yanfang, JIN Zhaohui, LI Tielong, KANG Haiyan, WANG Shuaima. [J]. Front.Environ.Sci.Eng., 2007, 1(3): 357-361.WU Deyi, HU Zhanbo, WANG Xinze, HE Shengbing, KONG Hainan. [J]. Front.Environ.Sci.Eng., 2007, 1(2): 213-220.XU Zhengyong, YANG Zhaohui, ZENG Guangming, XIAO Yong, DENG Jiuhua. [J]. Front.Environ.Sci.Eng., 2007, 1(1): 43-48.

关键词： ammonium     anoxygenic phototrophic bacterium     biohydrogen     glutamine auxotrophic     tofu wastewater    

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Improved energy recovery from dark fermented cane molasses using microbial fuel cells

Soumya Pandit, Balachandar G, Debabrata Das

《化学科学与工程前沿（英文）》 2014年 第8卷 第1期   页码 43-54 doi: 10.1007/s11705-014-1403-4

摘要： A major limitation associated with fermentative hydrogen production is the low substrate conversion efficiency. This limitation can be overcome by integrating the process with a microbial fuel cell (MFC) which converts the residual energy of the substrate to electricity. Studies were carried out to check the feasibility of this integration. Biohydrogen was produced from the fermentation of cane molasses in both batch and continuous modes. A maximum yield of about 8.23 mol H /kg COD was observed in the batch process compared to 11.6 mol H /kg COD in the continuous process. The spent fermentation media was then used as a substrate in an MFC for electricity generation. The MFC parameters such as the initial anolyte pH, the substrate concentration and the effect of pre-treatment were studied and optimized to maximize coulombic efficiency. Reductions in COD and total carbohydrates were about 85% and 88% respectively. A power output of 3.02 W/m was obtained with an anolyte pH of 7.5 using alkali pre-treated spent media. The results show that integrating a MFC with dark fermentation is a promising way to utilize the substrate energy.

关键词： dark fermentation     biohydrogen     microbial fuel cell     volatile fatty acid     anolyte    

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Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

《环境科学与工程前沿（英文）》 2011年 第5卷 第4期   页码 519-525 doi: 10.1007/s11783-011-0326-2

摘要： If cellulose can be effectively hydrolyzed into glucose by cellulase, the production costs of hydrogen, ethanol or other chemicals from cellulosic materials will be greatly decreased, and economically viable production of biohydrogen and bioethanol will become feasible. Cellulose is degraded into glucoses by multi-component enzyme systems. Nowadays cellulases are widely used in brewing, food, bioenergy, fodder, textiles, paper, pharmaceuticals, environmental protection and other industries. However, existing cellulases have several problems that limit their wider applications, including the low turnover number for solid cellulosic materials, and low stability in adapting to various application conditions. For example, high temperature, low pH, and so on. Application of directed evolution technology may be one of the most effective ways for improving the characteristics of cellulases. This paper presents a brief review of the cellulose hydrolysis mechanism by cellulase, advances in cellulases (endoglucanase and -glucosidase) improvement by directed evolution for several characteristics (for instance, thermal stability, pH adaptability and enzyme activity), limitations of directed evolution for cellulases, and the outlook for directed evolution for cellulase.

关键词： biohydrogen     bioethanol     cellulase     cellulose     directed evolution    

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“Composition” and regularisation of architectural production in contemporary China

Hua LI

《结构与土木工程前沿（英文）》 2010年 第4卷 第4期   页码 465-473 doi: 10.1007/s11709-010-0097-z

摘要： The purpose of this paper is to explore how the “Beaux-Arts” “composition” sustained the constitution of design principles for architectural production in China in the last two decades of the twentieth century. “Composition”, a key technique of the “Beaux-Arts” system, is not a simple act of “putting elements together” into a unified whole, but also relates to an array of different “constructions” of architectural knowledge which regulate and sustain the act of design. Through analysing the structure of three “textbooks” published in the early 1980s, this paper examines three aspects of the “constructions”: the setting up of the principles for governing architectural forms, regularisation of design methods and approaches for architectural practice, and the construction of a “tradition” for the expression of “Chineseness”.

关键词： composition     “Beaux-Arts”     regulate     architectural production    

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A comparison of production system life cycle models

Rajesh ATTRI, Sandeep GROVER

《机械工程前沿（英文）》 2012年 第7卷 第3期   页码 305-311 doi: 10.1007/s11465-012-0332-5

摘要：

Companies today need to keep up with the rapidly changing market conditions to stay competitive. The main issues in this paper are related to a company’s market and its competitors. The prediction of market behavior is helpful for a manufacturing enterprise to build efficient production systems. However, these predictions are usually not reliable. A production system is required to adapt to changing markets, but such requirement entails higher cost. Hence, analyzing different life cycle models of the production system is necessary. In this paper, different life cycle models of the production system are compared to evaluate the distinctive features and the limitations of each model. Furthermore, the difference between product life cycle and production life cycle is summarized, and the effect of product life cycle on production life cycle is explained. Finally, a production system life cycle model, along with key activities to be performed in each stage, is proposed specifically for the manufacturing sector.

关键词： production system     life cycle     model     product    

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Multiple Pollutants from Crop and Livestock Production in the Yangtze River: Status and Challenges

《农业科学与工程前沿（英文）》 doi: 10.15302/J-FASE-2023511

摘要：

● Cash crops and livestock production in Yangtze River Basin has grown rapidly.

关键词： Agriculture Green Development     crop production     livestock production     multi-pollutant models     multiple pollutants     Yangtze River Basin    

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EGG PRODUCTION IN CHINA: CURRENT STATUS AND OUTLOOK

《农业科学与工程前沿（英文）》 2021年 第8卷 第1期   页码 25-34

摘要：

• China is now the largest egg production country worldwide

• Egg production in China is characterized by diversity in several aspects

• China is now capable of breeding new varieties, with more than 50% of the market share

• Policies have been implemented to ensure sustainable development of egg production

• Integrating crop-chicken-vegetable production system is established

 

Eggs are one of the most nutritious and affordable animal products worldwide. From 1985, egg production in China has retained the leading place in the world. A total of 33 Mt of eggs were produced in 2019 representing ˃ 40% of the world total production. Egg production in China is characterized by diversity in several aspects, including layer breeds, products and production systems. New breeds and synthetic lines are developed to improve the genetic potentials of egg production and feed efficiency of layers. In the past, layer farms were run mostly by small households with 100 to 1000 layers per farm. Over the past decades, egg production in China has developed toward standardization and expansion of production systems, and many of these modern intensive farms raise millions of layers. Although the Chinese egg products maintain strong competitiveness over other animal products and imported egg products, the egg industry will grow at a slower pace compared to the past. Chinese consumers are more concerned about the quality and safety of eggs and egg products, as well as the environmental issues related to animal production, which presents challenges for the Chinese egg industry.

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Persistent Integration and Innovation Management Mode and Its Applications in Petroleum Production Engineering

He Liu

《工程管理前沿（英文）》 2015年 第2卷 第4期   页码 339-343 doi: 10.15302/J-FEM-2015055

摘要： This paper proposes a new management mode named the persistent integration and innovation management mode to satisfy the requirements by the fast development at oilfields. Taking engineering philosophy as the guidance and technical innovations in petroleum production engineering as the breaking point, the management mode combines long term production practice at oilfields with modern management methods. The management mode integrates intellectuals, techniques, capital, and information and so on to accomplish global optimization of resource configuration. Sets of new techniques, new methods, new technologies and new equipment in petroleum production engineering have been proposed under the guidance of the management mode, achieving great social and economic benefits.

关键词： integration and innovation     engineering philosophy     petroleum production engineering     Daqing Oilfield    

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标题 作者 时间 类型 操作

Continuous biohydrogen production from diluted molasses in an anaerobic contact reactor

Sheng CHANG, Jianzheng LI, Feng LIU

期刊论文

Optimization and modeling of biohydrogen production by mixed bacterial cultures from raw cassava starch

Shaojie Wang,Zhihong Ma,Ting Zhang,Meidan Bao,Haijia Su

期刊论文

Effects of nitrate concentration on biological hydrogen production by mixed cultures

Bo WANG, Wei WAN, Jianlong WANG,

期刊论文

Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus

LI Yongfeng, REN Nanqi, LI Jianzheng, LI Peng, YANG Chuanping

期刊论文

Effect of illumination on the hydrogen-production capability of anaerobic activated sludge

Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO

期刊论文

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ

期刊论文

Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter

Guanghong ZHENG, Zhuhui KANG, Yifan QIAN, Lei WANG,

期刊论文

Improved energy recovery from dark fermented cane molasses using microbial fuel cells

Soumya Pandit, Balachandar G, Debabrata Das

期刊论文

Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

期刊论文

Reiner Anderl：Smart Product and Production Engineering enabling new Business Models（2019年9月18日）

2021年08月12日

会议视频

“Composition” and regularisation of architectural production in contemporary China

Hua LI

期刊论文

A comparison of production system life cycle models

Rajesh ATTRI, Sandeep GROVER

期刊论文

Multiple Pollutants from Crop and Livestock Production in the Yangtze River: Status and Challenges

期刊论文

EGG PRODUCTION IN CHINA: CURRENT STATUS AND OUTLOOK

期刊论文

Persistent Integration and Innovation Management Mode and Its Applications in Petroleum Production Engineering

He Liu

期刊论文